Improvement of Large-Scale Production of Lignocellulosic Bioethanol through Synthetic Biology Approaches: A Comprehensive Review
نویسندگان
چکیده
This review article explores the use of synthetic biology approaches like gene editing and metabolic engineering to create genetically-modified organisms that can produce commercially-valuable products on large-scale, clean environmental pollutants, serve as efficient biosensors. The Biobrick system includes a registry available genetic information set standard protocols for assembling testing them, which finds various significant uses in biology, such production "Fourth generation” biofuel. Genetic techniques may be applied manipulate crops or microbes more biofuel production. Such also include modification lignin structure content, bacterial enzymes abundantly expressed plants, manipulation hemicellulose biosynthesis. However, challenges high temperature, conversion all substrate molecules maximum product yield, pentose fermentation, tolerance acetic acid bioethanol concentration impact yield product. innovative designed decrease levels enhance crop digestibility with specific focus model plant Arabidopsis thaliana herbaceous plants Alfalfa. aim is Monolignol pathway modify CSE gene, known its ability content. These modifications have been shown significantly improve saccharification efficiency increase lignocellulosic bioethanol. Besides, role xylulose-5-phosphate an intermediate non-oxidative phosphate oxidative pathway, heterologous xylose transporters modified sugar potential solutions discussed this article.
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ژورنال
عنوان ژورنال: World Journal of Biology Pharmacy and Health Sciences
سال: 2023
ISSN: ['2582-5542']
DOI: https://doi.org/10.30574/wjbphs.2023.14.3.0275